About

DBT - SAHAJ Viral Bioassay Facility (VBaF) at the Institute of Advanced Virology (IAV) is a national facility supported under the Department of Biotechnology DBT- SAHAJ ((DBT-SAHAJ) program. It offers the highest quality viral assays and therapeutic services to industry and academia thereby positively contributing to public health. The VBaF services include high-throughput BSL-2 neutralizing antibody/antiviral screening assays, a Viral Antigen production facility, and a platform facility for Recombinant monoclonal antibodies against emerging viruses.

Contact : vbaf@iav.res.in

Telephone: +91-471-2710067

 

 

 

LIST OF EQUIPMENT

Automated Protein Purification System (NGC DISCOVER 100 PLUS)

Applications and Uses of Medium-Pressure Chromatography Systems

  • Preparative chromatography
  • Protein purification and isolation

Features and Benefits of the NGC Discover 100 System

  • Automated 100 ml/min pumps provide accurate gradients for high-resolution separation of biomolecules
  • Automated sample injection of large sample volumes using fixed or dynamic loops
  • Gradient separations at different pH values and buffer pH monitoring, for rapid method development
  • Automated buffer and salt gradients with buffer blending during automated inline buffer preparation at flow rates of up to 200 ml/min, for rapid scouting of purification conditions
  • Simultaneous four-wavelength detection of proteins, peptides, nucleic acids, and chromophores with conductivity measurement and pH detection
  • Compatibility with the NGC Fraction Collector and BioFrac Fraction Collector for sample collection

Floor Mount Fermenter (JENIK Hyperion SS) 

High Flexible floor mounting labscale fermenter suitable for research and Industry for both Microbial and cell culture application. Ideal system for trouble free operation. Well equipped for batch, fed batch & continuous. Flexible control system for customized function.

Features and benefits

  • Space-saving Design-Takes up minimal bench space
  • Feeding System-High precise feeding system integrated with feeding rack & bottles
  • Multiple Control-Operate multiple reactors independently
  • Efficient Heating-Jacketed heating for precise temperature control
  • Scalable- Multiple skid integration
  • Data Management-Complete process measurement and data handling

ELISPOT (S6 Ultimate M2)

It enables advanced 12-color fluorescent work, in addition to 12 color bright field studies, live-dead-apoptotic cell counting, and histological analysis. It is also ideally suited for multi-color viral neutralization and infectivity assays as well as differential bacterial colony counting. A powerful zoom lens not only affords optical resolution suited for multi-color single-cell analysis but enables high resolution work in any well format. Its modular design permits customization for optimizing many imaging applications. It comes loaded with software supporting a wide range of assays. This instrument is high-throughput capable handling up to 400 plates per run when equipped with our automated plate loader. It has been designed for professionals working in the regulated or academic field.

Applications

Single-Color ELISPOT, Double-Color Enzymatic ELISPOT, Twelve-Color FluoroSpot, Twelve-Color Fluorescence BioSpot, FRNT / FRA PRNT / Viral Titer Assays, Bacterial/Yeast/Viral Colony Counting, Live/Dead/Apoptotic PBMC Counting, Twelve-Color Microscopic Fluorescent Cell Imaging, Cell-Mediated Cytotoxicity Assays, Histology Slide Analysis, ELISA Analysis, Proficiency Testing, Suited for Regulatory Compliance, Suited for BSL 3/4.