Plaque reduction-based Antibody Assay Services.
The Viral Bioassay facility (VBaf) offers antibody screening assay services to validate the virus inhibition potential of your antibodies/antiviral compounds. The assay is based on the Plaque Reduction Neutralization Test (PRNT) which measures the ability of the antibodies/antiviral compounds to neutralize a virus. The antibodies already validated to show antiviral activity against the virus of interest will be used as an internal control.
Assay format and service catalogue details
| Catalogue No. | Service Name | Plate Type | Dilution Scheme | Cost per sample |
| PRNT-DV1-24W-8D | PRNT Assay – Dengue Virus Serotype 1 | 24-well plate | 8-point serial dilution in duplicates | Rs. 25,000 + 18% GST |
| PRNT-DV2-24W-8D | PRNT Assay – Dengue Virus Serotype 2 | 24-well plate | 8-point serial dilution in duplicates | Rs. 25,000 + 18% GST |
| PRNT-DV3-24W-8D | PRNT Assay – Dengue Virus Serotype 3 | 24-well plate | 8-point serial dilution in duplicates | Rs. 25,000 + 18% GST |
| PRNT-DV4-24W-8D | PRNT Assay – Dengue Virus Serotype 4 | 24-well plate | 8-point serial dilution in duplicates | Rs. 25,000 + 18% GST |
| PRNT-CHIKV-24W-8D | PRNT Assay – Chikungunya Virus | 24-well plate | 8-point serial dilution in duplicates | Rs. 20,000 + 18% GST |
Deliverables
- IC50 values with interpretation
- Raw plaque count data
- Summary report (PDF)
- Representative images
Notes:
- All samples must be submitted with a completed submission form.
- Repeat or customized assays can be arranged upon request
- Payment for the samples should be made in advance. The account details for payment is as below:
| Account no | 5513101003216 |
| IFSC Code | CNRB0005513 |
| Bank | Canara Bank |