Pseudovirus-based Neutralization Test (PVNT)
The Pseudovirus-based Neutralization Test (PVNT) has emerged as a robust and versatile alternative to traditional virus neutralization assays in modern virology. By utilizing recombinant viral vectors (such as Lentivirus or VSV) engineered to display the surface glycoproteins of highly pathogenic viruses, PVNT allows for the precise measurement of neutralizing antibodies without the need for the live, infectious agent. This system provides a safer, high-throughput platform that can be conducted in Biosafety Level 2 (BSL-2) facilities, significantly accelerating vaccine and antibody evaluation studies.
Assay format and service catalogue details
| Catalogue No. | Service Name | Plate Type | Dilution Scheme | Cost per sample |
| PVNT-NiV-I* | PVNT Assay – Nipah Virus | 96-well plate | 8-point serial dilution in triplicates | Rs. 25,000 + 18% GST |
| PVNT-NiV-II** | PVNT Assay – Nipah Virus | 96-well plate | Single dilutions upto 72 samples | Rs. 30,000 + 18% GST |
| PVNT-RBV-I* | PVNT Assay – Rabies Virus | 96-well plate | 8-point serial dilution in triplicates | Rs. 25,000 + 18% GST |
| PVNT-RBV-II** | PVNT Assay – Rabies Virus | 96-well plate | Single dilutions upto 72 samples | Rs. 30,000 + 18% GST |
Deliverables
- *IC50 values with interpretation
- *Summary report (PDF)
- **Activity report only (PDF)
Notes:
- All samples must be submitted with a completed submission form.
- Repeat or customized assays can be arranged upon request
- Payment for the samples should be made in advance. The account details for payment is as below:
| Account no | 5513101003216 |
| IFSC Code | CNRB0005513 |
| Bank | Canara Bank |
Anti Rabies Antibodies in Human Serum (Clinical) - Sample Submission form